Short Course Description
Antibody Engineering 0453-3388
The course is a series of 13 (in a regular year) 2-hr lectures that provide an updated view of the fascinating world of Antibody Engineering.
The curriculum includes: An introduction to Antibody Engineering ? What are antibodies? why are the important biologically and commercially? why do we engineer antibodies? Antibodies structure and function: from genetic organization to 3D structure. The contribution of Bioinformatics to Antibody Engineering. Systems for antibody cloning and expression. Antibody humanization. Advanced antibody discovery approaches: transgenic animals, Display technologies, Next-generation sequencing and proteomics. Medical and Biotechnological applications of recombinant antibodies: naked and armed antibodies.
First Semester. 2 credit points.
The final grade is based on a multiple-choice exam (3 hours) containing 40 questions.
Tentative list of lectures (some lectures take more than a single 2 hr class):
1) Introduction
2) Generation of diversity
3) Armed antibodies
4) Antibody structure and Bioinformatics
5) Cloning antibody genes
6) Humanization of antibodies
7) Stabilization of antibodies
8) Antibody Developability
9) Antibody discovery using transgenic animals
10) Antibody phage display
11) Additional display technologies
12) Conclusions
Full syllabus will be available to registered students only
