Short Course Description
The course will cover a variety of methods for analyzing DNA, RNA, proteins, and cells. Some of the methods covered for DNA and RNA analysis are advanced cloning techniques, real-time PCR, digital droplet PCR, Southern and Northern blotting, DNA microarrays, next-generation sequencing, single-cell sequencing, in situ hybridization (FISH and CISH), and chromatin immunoprecipitation (ChIP). For protein analysis: Western blotting, immunoprecipitation, ELISA (enzyme-linked immunosorbent assay), and immunofluorescence. For cell analysis and microscopy: FACS analysis, immunofluorescence, live cell imaging, confocal microscopy, TIRF, super-resolution (STED, STORM), SNAP/Halo-tag, FRAP, FRET, basics of electron microscopy, and fluorescence-EM correlation. The course will also include exercises in the lecture schedule.
Full syllabus will be available to registered students only
